Description

• General description: Ammonium sulfate is naturally present as a rare mineral mascagnite in volcanic fumaroles and due to coal fires in some dumps.
• Application: Ammonium sulfate has been used:to prepare mobile phase A for hydrophobic interaction chromatography (HIC)as reference material for the thermal decomposition of NH4NO3 (S1–S6) thermal decomposition to N2O (S1-N2O–S6-N2O)to prepare the stock solution for M63 mediumas a reagent in molecular biology and chromatographyin the precipitation and fractionation of proteinsin the purification of antibodiesfor the crystallographic analysis of nucleic acids and proteinsin high-performance liquid chromatography (HPLC) analysis of proteins, such as in hydrophobic interaction chromatographyfor Sepharose chromatography of tRNAs using reverse salt gradientsin long PCR buffer, in PCR lysis solution, and second-strand cDNA synthesisHPLC analysis of phospholipids
• Biochem/physiol Actions: Ammonium sulfate is an inorganic salt, which is mostly used as a soil fertilizer. It is also used as a flame retardant since it elevates the combustion temperature of the material and lowers maximum weight loss rates. This leads to an upsurge in the production of residue or char.
• Other Notes: The most commonly used salt for the fractional precipitation of proteins due to its solubility and effectiveness[1]; Protein crystallization[2]; Sepharose™ chromatography of tRNAs using reverse salt gradients[3].
• Legal Information: Sepharose is a trademark of Cytiva