7089814
TSKgel™ Boronate-5PW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™
High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins.
Manufacturer: Fischer Scientific
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Flow Rate
1.2 mL/min
Particle Size
10 μm
Solvents
≤ 20% Organic Solvent
Stationary Phase
M-Aminophenyl Boronate
Sample Type
Proteins
Technique
Affinity
Max. Pressure
30 bar
pH
2 to 9
Temperature
>0°C
Product Line
TSKgel
Matrix
M-Aminophenyl Boronate Phase/Hydroxylated Methacrylic Polymer
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Description
- High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds, and catecholamines Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers Adsorption onto the matrix takes place in basic buffers such as HEPES and morpholine Desorption takes place in carbohydrate or amine-containing mobile phases such as sorbitol or Tris Resin Adsorption Capacity: 40 μmol/mL