4521739
Thermo Scientific™ Eco47III (AfeI) (10 U/μL)
The Eco47III (AfeI) restriction enzyme recognizes AGC^GCT sites and cuts best at 37°C in O buffer (Isoschizomers: AfeI, Aor51HI).
Manufacturer: Fischer Scientific
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Concentration
10 U/μL
Enzyme
Eco47III (AfeI)
Sensitive to Heat Inactivation
Yes
Type IIS RE
No
Research Category
Traditional Cloning
Methylation Sensitivity
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer
10X Buffer O
Optimal Reaction Temperature
37°C
Product Type
Restriction Enzyme
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Description
- Lambda DNA digested with Eco47III (AfeI), 0.7% agarose, 2 cleavage sites conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System
- In addition, the universal Tango buffer is provided for convenience in double digestions
- All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions
- To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations
- Highlights Superior quality -stringent quality control and industry leading manufacturing process Convenient color-coded Five Buffer System Includes universal Tango buffer for double-digestions BSA premixed in reaction buffers Wide selection of restriction endonuclease specificities