4521795

Thermo Scientific™ HinfI, HC (50 U/μL)

The HinfI restriction enzyme recognizes G^ANTC sites and cuts best at 37°C in R buffer.

Manufacturer: Fischer Scientific

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Concentration

10 U/μL

Enzyme

Hinf I

Sensitive to Heat Inactivation

Yes

Type IIS RE

No

Research Category

Traditional Cloning

Methylation Sensitivity

CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive

Compatible Buffer

10X Buffer R

Optimal Reaction Temperature

37°C

Product Type

Restriction Enzyme

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Description

  • Lambda DNA digested with HinfI, 1.4% agarose, 148 cleavage sites conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System
  • In addition, the universal Tango buffer is provided for convenience in double digestions
  • All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions
  • To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations
  • Highlights Superior quality -stringent quality control and industry leading manufacturing process Convenient color-coded Five Buffer System Includes universal Tango buffer for double-digestions BSA premixed in reaction buffers Wide selection of restriction endonuclease specificities