4521809

Thermo Scientific™ GsuI (BpmI) (5 U/μL)

The GsuI (BpmI) restriction enzyme recognizes CTGGAG(16/14)^ sites and cuts best at 30°C in B buffer (Isoschizomers: BpmI).

Manufacturer: Fischer Scientific

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Concentration

5 U/μL

Enzyme

GsuI (BpmI)

Sensitive to Heat Inactivation

Yes

Type IIS RE

Yes

Research Category

Scarless Cloning

Methylation Sensitivity

Dcm Methylation-Sensitive, Not Dam Methylation-Sensitive, Not CpG Methylation-Sensitive

Compatible Buffer

10X Buffer B

Optimal Reaction Temperature

30°C

Product Type

Restriction Enzyme

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Description

  • Lambda DNA digested with GsuI (BpmI), 1% agarose, 25 cleavage sites conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System
  • In addition, the universal Tango buffer is provided for convenience in double digestions
  • All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions
  • To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations
  • Highlights Superior quality -stringent quality control and industry leading manufacturing process Convenient color-coded Five Buffer System Includes universal Tango buffer for double-digestions BSA premixed in reaction buffers Wide selection of restriction endonuclease specificities