4522088
Thermo Scientific™ TasI (Tsp509I) (10 U/μL)
Cut at ^AATT sites with TasI (Tsp509I) restriction enzyme, which performs best at 65°C in B buffer (Isoschizomers: Sse9I, Tsp509I, TspEI).
Manufacturer: Fischer Scientific
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Concentration
10 U/μL
Enzyme
TasI (Tsp509I)
Sensitive to Heat Inactivation
No
Type IIS RE
No
Research Category
Traditional Cloning
Methylation Sensitivity
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer
10X Buffer B
Optimal Reaction Temperature
65°C
Product Type
Restriction Enzyme
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Description
- Lambda DNA digested with TasI (Tsp509I), 1% agarose, 189 cleavage sites conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System
- In addition, the universal Tango buffer is provided for convenience in double digestions
- All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions
- To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations
- Highlights Superior quality -stringent quality control and industry leading manufacturing process Convenient color-coded Five Buffer System Includes universal Tango buffer for double-digestions BSA premixed in reaction buffers Wide selection of restriction endonuclease specificities