FERER0633
Thermo Scientific™ PvuII, HC (50 U/μL)
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERER0633-Each-of-1 | In Stock | ₹ 17,613.10 |
FERER0633 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,613.10
GST (18%): ₹ 3,170.358
Total Price: ₹ 20,783.458
Concentration
50 U/μL
Enzyme
Pvu II
Sensitive to Heat Inactivation
No
Type IIS RE
No
Product Type
Restriction Enzyme
Methylation Sensitivity
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer
10X Buffer G
Optimal Reaction Temperature
37°C
Quantity
12,500 U
Research Category
Traditional Cloning
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Description
- Thermo Scientific™ PvuII is available as a FastDigest enzyme which is used for rapid DNA digestion, more than 90% of the digested DNA fragments can be ligated and more than 95% of these sites can be recut at a 5'-termini concentration of 0.15 μM after a 10-fold overdigestion (5U/μg DNA x 2 hours) with PvuII
- 5'...C A G▵C T G...3' 3'...G T C▵G A C...5' Conditions for 100% Activity 1X Buffer G:10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl 2 , 50mM NaCl and 0.1mg/mL BSA Incubate at 37°C Storage Buffer PvuII is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol Ligation and Recleavage After 10-fold overdigestion with PvuII, more than 90% of the DNA fragments can be ligated and more than 95% of these can be recut Methylation Effects Dam: never overlaps —no effect Dcm: never overlaps —no effect CpG: never overlaps —no effect EcoKI: never overlaps —no effect EcoBI: may overlap —effect not determined Digestion of Agarose-embedded DNA Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours Note: Greater than 15-fold overdigestion with PvuII may result in star activity.