Thermo Scientific™ GeneJET Plasmid Miniprep Kit

Description

• GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column
• The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure
• Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column
• The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure
• Highlights • Efficient —high yields of up to 20 μg of high quality plasmid DNA • Fast —procedure takes less than 14 minutes • Convenient —no phenol/chloroform extraction or alcohol precipitation required • Pure —purified DNA is immediately ready to use Applications • Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including: • FastDigest or conventional restriction digestion • Automated fluorescent and radioactive sequencing • PCR • In vitro transcription • Transformation Principle A bacterial culture is harvested and lysed
• The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration
• The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water
• The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing
• Components Resuspension Solution Lysis Solution Neutralization Solution Wash Solution (concentrated) RNase A Solution Elution Buffer Spin Columns Collection Tubes Detailed Protocol