A11153

Invitrogen™ PichiaPink™ Secreted Vector Kit

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 A11153-Each-of-1 In Stock ₹ 1,23,354.00

A11153 - Each of 1

₹ 1,23,354.00

In Stock

Quantity

1

Base Price: ₹ 1,23,354.00

GST (18%): ₹ 22,203.72

Total Price: ₹ 1,45,557.72

Format

Liquid

Promoter

AOX1

Content And Storage

PichiaPink™ Secreted Protein Vector kit contains 20 μg pPinkα-HC (the high-copy number plasmid with the S. cerevisiae Alpha mating factor presequence) with sequencing primers 5' α-factor and 3' CYC1. Store at -20°C.

Cleavage

No Cleavage Site

Shipping Condition

Room Temperature

Cloning Method

Restriction Enzyme/MCS

Inducing Agent

Methanol

Product Type

Vector Kit

Antibiotic Resistance Bacterial

Ampicillin (AmpR)

Replication Origin

pUC Origin

Protein Tag

Untagged

Vector

pPink

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Description

  • The PichiaPink™ Secreted Vector Kit contains the pPINKα-HC vector for use with the PichiaPink™ Yeast Expression System
  • The pPINKα-HC vector is built around the ADE2 gene for complementing the complementing the ade2 deletion in the PichiaPink™ strains
  • The pPINKα-HC vector has the α-mating factor secretion signal sequence so that all proteins are secreted
  • You can order the PichiaPink™ Secreted Vector Kit to refill your PichiaPink™ Yeast Expression System Kit
  • The PichiaPink™ Secreted Vector Kit comes with: pPINKα-HC vector (high-copy number) (20μg) 5’α-factor sequencing primer (20μg) 3’CYC1 sequencing primer (20μg) One Shot™ TOP10™ Electrocomp™ E
  • coli (Cat
  • No
  • C404052) Why Choose the PichiaPink™ Yeast Expression System? The PichiaPink™ Yeast Expression System is based on the yeast Pichia pastoris
  • Advantages of Pichia pastoris include rapid growth, well-defined genetic background, simple media formulation, and easy handling
  • For over 30 years, Pichia pastoris has been used by labs around the world for producing hundreds of different proteins from many species including human (Ref 1, 2)
  • The PichiaPink™ Yeast Expression System allows convenient and cost-effective protein production from small to large scales
  • For information on obtaining a commercial-use license for the PichiaPink™Yeast Expression System, please inquire at outlicensing@lifetech.com
  • For Research Use Only
  • Not intended for any animal or human therapeutic or diagnostic use
  • Related Links Download the pPINKα-HC plasmid map (PDF)
  • Learn more about the PichiaPink™ Yeast Expression System
  • Learn more about our other protein expression systems
  • References 1
  • Cereghino JL, Cregg JM
  • Heterologous protein expression in the methylotrophic yeast Pichia pastoris
  • FEMS Microbiol Rev
  • 2000 Jan;24(1):45-66
  • [ PubMed ] 2
  • Cereghino GP, Cereghino JL, Ilgen C, Cregg JM
  • Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris
  • Curr Opin Biotechnol
  • 2002 Aug;13(4):329-32
  • [ PubMed ]