Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K480040-Each-of-1 | In Stock | ₹ 1,87,790.00 |
K480040 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,87,790.00
GST (18%): ₹ 33,802.20
Total Price: ₹ 2,21,592.20
Constitutive or Inducible System
Constitutive
Promoter
CMV
Selection Agent (Eukaryotic)
Geneticin™ (G-418)
Protein Tag
His Tag (6x), V5 Epitope Tag
Quantity
40 Reactions
Product Line
TOPO™, pcDNA™
Delivery Type
Transfection
Product Type
TOPO TA Expression Kit
Content And Storage
2 × Box 1 (store at -20°C)• 20 μl pcDNA™3.1/V5-His-TOPO™ (10 ng/μl)• 100 μl 10X PCR Buffer• 10 μl dNTP Mix (50 mM)• 50 μl Salt Solution• 1 ml Sterile Water• 10 μl Control PCR Template (50 ng/μl)• 10 μl Control PCR Primers (100 ng/μl each)• 20 μl T7 Sequencing Primer (100 ng/μl)• 20 μl BGH Reverse Sequencing Primer (100 ng/μl)• 10 μl Expression Control Plasmid (500 ng/μl)2 × Box 2 (store at -80°C)• 21 × 50 μl TOP10E. colicells• 50 μl pUC19 Control DNA (10 pg/μl)• 6 ml S.O.C. Medium
Cloning Method
TOPO-TA
Vector
TOPO-TA Vectors, pcDNA
For Use With (Application)
Constitutive Expression
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Description
- No ligase, post-PCR procedures, or PCR primers containing specific sequences are required Once cloned, analyzed, and transfected, PCR product will express directly in mammalian cell lines pcDNA3.1/V5-His TOPO vector features strong CMV promoter for high-level, constitutive expression C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with Anti-His (C-term)antibody Topoisomerase activation of pcDNA3.1/V5-His-TOPO vector allows PCR products to be ligated in 5 minutes on bench top and results in 90% recombinants Vector features strong CMV promoter for high-level, constitutive expression C-terminal V5 epitope tag for efficient detection of recombinant proteins with Anti-V5 antibody Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis Order Info Shipping Condition: Dry Ice