69-451-3
MilliporeSigma™ Novagen™ BL21(DE3) pLysS Competent Cells
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 69-451-3-Each-of-1 | In Stock | ₹ 16,642.11 |
69-451-3 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,642.11
GST (18%): ₹ 2,995.58
Total Price: ₹ 19,637.69
Efficiency
Guaranteed efficiency: >2 x 107cfu/μg
Content And Storage
≤-70°C
Quantity
0.4 mL
Related Products
Description
- BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases
- DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter
- Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG
- pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.