4518347
Cytiva Ni Sepharose™ excel
Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Manufacturer: Fischer Scientific
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Buffer
0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
Flow Rate
150 to 600cm/hr.
Particle Size
90μm
Content And Storage
4° to 30°C
Product Type
Ni Sepharose excel chromato graphy media
pH Range
2 to 12
Support Type
6% Highly Cross-linked Agarose
Matrix
Ni-Sepharose
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Description
- Ni Sepharose excel enables direct loading of samples without having to perform extensive and time-consuming pretreatment procedures
- Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
- Increase target protein yield and decrease degradation through reduced and simplified sample handling
- Choose between several formats for screening and preparative purification of histidine-tagged proteins
- Ni Sepharose excel is an IMAC medium precharged with nickel ions very strongly bound to a chelating ligand, allowing loading of samples that usually cause stripping of metal ions
- Ni Sepharose excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.