Anti-Integrin α6 Antibody, clone MA6

Description

• Specificity: Reacts with the laminin receptor alpha-6beta-1 and alpha-6beta-4 complexes.
• Application: Immunoprecipitation, flow cytometry, immunohistochemistry in frozen tissue sections. Product does not detect alpha 6 in Western blot since the epitope most likely involves both alpha 6 and beta 1 integrin subunit. Product does not block alpha 6 integrin mediated cell adhesion to laminin. Activation of integrin signaling properties by MA6 has not been characterized. Immunoprecipitation of VLA-6 from lysate of 10E6 cell equivalent requires 2 μg of MA6. Recommended concentration for flow cytometry - 10 μg/mL, staining of frozen tissue sections - 20 μg/mL. Optimal working dilutions must be determined by end user. Cell lines tested by using MA6 to express (6 integrins include: mouse bone marrow-derived mast cells, melanoma cell line B16F1, squamous carcinoma SCC-VII, fibroblast cell line NIH-3T3, splenic T lymphocytes, thymocytes and various T cell lines.
• Physical form: Format: Purified
• Storage and Stability: Store at +2-8°C for up to 12 months.
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany