ROR gamma (t) Monoclonal Antibody (AFKJS-9), eBioscience™, Invitrogen™

Description

• The AFKJS-9 monoclonal antibody reacts with the mouse, human and rhesus monkey ROR gamma (t) protein
• ROR gamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR alpha and ROR beta
• ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes
• ROR gamma is expressed in several tissues including liver, lung, muscle, heart and kidney
• Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR gamma (t), which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ double-positive thymocytes, Th17 cells of the periphery and lymphoid tissue inducer (LTi) cells of lymphoid organs
• The ROR gamma (t) isoform differs from ROR gamma by three unique amino acids at its amino terminus
• Therefore, the AFKJS-9 antibody will react with both the ROR gamma and ROR gamma (t) isoforms
• Applications Reported: This AFKJS-9 antibody has been reported for use in chromatin immunoprecipitation and western blotting
• Applications Tested: This AFKJS-9 antibody has been tested by western blot analysis
• This can be used at less than or equal to 5 μg per mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis
• ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L)
• ROR gamma is also required for the development of lymph nodes and Peyer's patches
• It has been shown that ROR gamma(t), a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes
• ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence
• ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle
• ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas
• ROR gammat t (RORgt) is an isoform of RORC.