Desmoglein 2 Monoclonal Antibody (CSTEM28), eBioscience™, Invitrogen™

Description

• This monoclonal antibody CSTEM28 reacts with human desmoglein 2 (DSG2), a type 1 transmembrane protein belonging to the cadherin family
• DSG2 is expressed by epithelial cells, cardiomyocytes, endothelial progenitors, and CD34+CD45dim hematopoietic stem and progenitor cells
• This adhesion molecule forms cell-cell adhesion complexes called desmosomes that provide tensile strength to tissues that experience mechanical stress
• Other than its role in desmosome formation, DSG2 plays a role in barrier integrity, adhesion, migration, cell apoptosis, proliferation, vasculogenic mimicry, and intracellular signaling
• DSG2-expressing endothelial progenitor cells are pro-angiogenic, suggesting a role in vasculature formation
• On hematopoietic stem and progenitor cells, DSG2 expression is highest on CD38-CD90+ multi-potent cells
• DSG2 expression is progressively decreased as a function of differentiation, with DSG2 expression absent on mature lymphocytes
• DSG2 also serves as a receptor for adenoviruses and can be shed by mucosal barriers in the intestine
• This CSTEM28 antibody has been tested by flow cytometric analysis of the 2102ep cell line
• This has also been tested by immunocytochemistry of fixed and permeabilized cells and can be used at less than or equal to 10 μg/mL
• It is recommended the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types
• Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily
• These desmoglein gene family members are located in a cluster on chromosome 18
• This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris.