alpha-Fetoprotein Monoclonal Antibody (1E8), eBioscience™, Invitrogen™

Description

• Description: This 1E8 monoclonal antibody reacts with human alpha-fetoprotein (AFP)
• This 70-kDa secretory protein is a member of the albumin gene family
• Synthesized by the yolk sac and fetal liver during embryogenesis, AFP protein levels are highest in fetal serum
• After birth, serum AFP levels decrease dramatically
• In fact, AFP is nearly undetectable in normal adult serum
• However, hepatocellular carcinoma and germ cell teratoblastoma, as well as liver regeneration, viral hepatitis, and cirrhosis, leads to elevated AFP serum levels in adults
• As such, detection of this protein is frequently used as a diagnostic marker for these conditions
• Applications Reported: This 1E8 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry
• Applications Tested: This 1E8 antibody has been tested by western blot on reduced HepG2 cell lysate and by immunohistochemistry on FFPE human liver section using high pH antigen retrieval
• The antibody can be used at less than or equal to 5 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract and the liver during fetal life
• AFP expression in adults is often associated with hepatoma or teratoma
• However, hereditary persistence of AFP may also be found in individuals with no obvious pathology
• The protein is thought to be the fetal counterpart of serum albumin, and AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4
• AFP is found in monomeric, dimeric, and trimeric forms
• AFP can also bind to copper ions, nickel ions, fatty acids and bilirubin
• The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly
• High level of serum AFP has been identified in patients with hepatocellular carcinomas (HCC), teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.