CD122 Monoclonal Antibody (TM-b1 (TM-beta1)), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The TM-b1 monoclonal antibody reacts with mouse CD122, the 90-110 kDa interleukin-2 receptor beta chain (IL-2R beta)
• CD122 is expressed by NK cells and some T cells in the periphery, and is upregulated by activation
• CD122 associates with CD25 and CD132 (common gamma chain) to form the high affinity IL-2 receptor
• High affinity binding of IL-2 to the IL-2 receptor is inhibited by the TM-b1 antibody
• Applications Reported: This TM-b1 (TM-beta1) antibody has been reported for use in flow cytometric analysis
• It has also been reported to block high affinity binding of IL-2 to its receptor
• Applications Tested: The TM-b1 (TM-beta1) antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• CD122 (Interleukin 2 receptor) is involved in T cell-mediated immune responses, primarily expressed in the hematopoietic system and is present in 3 forms
• The low affinity form of CD122 is a monomer of the alpha subunit and is not involved in signal transduction
• The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer
• Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from CD122
• The protein encoded by the CD122 gene represents the beta subunit and is a type I membrane protein
• Diseases associate with CD122 protein dysfunction include oligoarticular juvenile idiopathic arthritis and rheumatoid factor negative juvenile idiopathic arthritis.