37-290-0
6x-His Tag Monoclonal Antibody (4A12E4), Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 37-290-0-Each-of-1 | In Stock | ₹ 40,050.00 |
37-290-0 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,050.00
GST (18%): ₹ 7,209.00
Total Price: ₹ 47,259.00
Antigen
6x-His Tag
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.1% sodium azide; pH 7.4
Host Species
Mouse
Purification Method
Protein A
Regulatory Status
RUO
Target Species
Tag
Product Type
Antibody
Isotype
IgG1 κ
Applications
Immunocytochemistry, Western Blot
Clone
4A12E4
Conjugate
Unconjugated
Gene Alias
6His tag; 6X His; His Tag; Histidine Tag
Immunogen
Synthetic peptide containing six His residues (6xHis).
Quantity
100 μg
Primary or Secondary
Primary
Content And Storage
-20°C
Form
Liquid
Target Molecule
Tag
Related Products
Description
- The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH)
- The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography
- Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering
- By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available
- His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins
- For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent
- His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.