3851559
Rab5 Mouse, Unlabeled, Clone: 1, BD
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Rab5
Concentration
250μg/mL
Classification
Monoclonal
Host Species
Mouse
Research Discipline
Cell Biology
Formulation
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
Isotype
IgG2a
Purification Method
Affinity Purified
Clone
1
Applications
Western Blot
Conjugate
Unconjugated
Regulatory Status
RUO
Target Species
Canine, Human
Immunogen
Human Rab5 aa. 1-215
Primary or Secondary
Primary
Content And Storage
Store undiluted at -20°C.
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Description
- Rab5 is a low molecular weight GTP-binding protein that plays a role in endocytic vesicle traffic
- Like other Rab proteins, Rab5 has C-terminal cysteine residues that are post translationally modified by geranylgeranylation which is critical for its membrane targeting
- Rab5 is associated with early endosome and plasma membranes and evidence suggests that Rab5 regulates early endosome fusion
- The GTP/GDP cycle controls shuttling of Rab proteins between the cytosol and membranes
- In vitro, Rab5 proteins are removed from membranes by a GDP dissociation inhibitor protein (rabGDI) which leads to the formation of a cytosolic Rab5-rabGDI complex
- Rab5 may insert into membranes by a multistep process in a which a transient GDP-Rab5 intermediate is formed and converted into GTP-Rab5 that subsequently enters the acceptor membrane and releases rabGDI into the cytosol
- Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting