3969413
CD3 Mouse anti-Human, V450, Clone: UCHT1, BD
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD3
Applications
Flow Cytometry
Conjugate
Violet Fluor 450 (V450)
Regulatory Status
RUO
Target Species
Human
Immunogen
Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Clone
UCHT1
Classification
Monoclonal
Host Species
Mouse
Research Discipline
Immunology
Formulation
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Isotype
IgG1 κ
Purification Method
Affinity Purified
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Description
- Reacts with the human ε-chain, a 20kDa subunit of CD3/T cell antigen receptor complex found on 70%-80% of normal human peripheral blood lymphocytes and 60%-85% of thymocytes
- Studies from the HLDA Workshop show this antibody to be mitogenic when used in conjunction with pokeweed mitogen
- CD3 plays a role in signal transduction during antigen recognition
- UCHT1 antibody stains intracellular CD3 unlike the other CD3 clone, HIT3a, which stains only the extracellular CD3
- The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences
- It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450nm
- Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates
- Host Species: Mouse Clone: UCHT1 Isotype: IgG1 κ Species Reactivity: Human Flow Cytometry, Functional Assay