3971855
ROCK-I Mouse, Unlabeled, Clone: 46, BD
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
ROCK-I
Concentration
250μg/mL
Classification
Monoclonal
Host Species
Mouse
Research Discipline
Cell Biology
Formulation
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
Isotype
IgG1
Purification Method
Affinity Purified
Description
ROKβ
Clone
46
Applications
Western Blot
Conjugate
Unconjugated
Regulatory Status
RUO
Target Species
Human, Mouse, Rat
Immunogen
Mouse ROCK-I aa. 906-1012
Primary or Secondary
Primary
Content And Storage
Store undiluted at -20°C.
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Description
- ROCK-I is a Rho-associated serine/threonine kinase isozyme that mediates RhoA-induced assembly of focal adhesions and actin stress fibers
- It contains an N-terminal kinase domain, a central 600 amino acid long coiled-coil region, a C-terminal pleckstrin homology region (PH) and a Cys-rich zinc finger motif
- The ROCK-I kinase domain is approximately 90% identical to that of ROCK-II
- ROCK-I binds GTP-bound Rho through a Rho-binding domain (RBD)
- As a result, the kinase activity of ROCK-I is moderately stimulated
- The ROCK isozymes regulate cell contractility through phosphorylation of the myosin light chain
- This effect results from either the inhibition of the myosin phosphatase or by direct phosphorylation of the myosin light chain, thus bypassing the myosin light chain kinase
- In addition, ROCK-I activates the ubiquitously expressed Na-H exchanger (NHE1) via a number of mechanisms including RhoA
- NHE1 may mediate ROCK-I-induced changes in the actin cytoskeleton
- Therefore, ROCK-I plays an important role in the regulation of focal adhesion and stress fiber formation
- Host Species: Mouse Clone: 46 Isotype: IgG1 Species Reactivity: Mouse Immunogen: Mouse ROCK-I aa
- 906-1012 Formula Weight [Chemical]: 160kDa Immunofluorescence, Western Blotting