4325806
CD14 Mouse anti-Human, V500, Clone: M5E2, BD
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD14
Applications
Flow Cytometry
Conjugate
Violet Fluor 500 (V500)
Regulatory Status
RUO
Formulation
Aqueous buffered solution containing protein stabilizer, glycerol and ≤0.09% sodium azide.
Isotype
IgG2a κ
Purification Method
Affinity Purified
Description
LPS receptor; LPS-R; Myeloid cell-specific leucine-rich glycoprotein
Clone
M5E2
Classification
Monoclonal
Host Species
Mouse
Target Species
Human
Immunogen
Human CD14 Protein
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Description
- The M5E2 monoclonal antibody specifically binds to CD14, a 53–55 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein expressed at high levels on monocytes
- Additionally, the anti-CD14 antibody reacts with interfollicular macrophages, reticular dendritic cells, and some Langerhans cells
- CD14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, LPB
- The antibody is conjugated to BD Horizon™ V500, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences
- It is excited by the Violet laser with an Ex max of 415nm and Em Max at 500 nm
- BD Horizon V500 conjugates emit at a similar wavelength to Amcyan yet exhibit reduced spillover into the FITC channel
- For more information on BD Horizon V500, visit bdbiosciences.com/colors.