CD16 Mouse anti-Human, PE-CF594, Clone: 3G8, BD

Description

• The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (FcγRIII), a human NK cell-associated antigen
• CD16 is expressed on NK cells as well as macrophages and granulocytes
• Reports indicate that CD16 plays a role in signal transduction and NK cell activation
• The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes
• The 3G8 antibody is reported (Vossebeld et al ., 1997) to increase intracellular calcium levels in human neutrophils by interacting with bothFcγRIIa and FcγRIIIb molecules
• This antibody has also been reported to induce homotypic neutrophil aggregation
• This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red
• PE-CF594 excites and emits at similar wavelengths to PE-Texas Red yet exhibits improved brightness and spectral characteristics
• Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red (eg 610/20-nm filter).