4697134
CD14 Mouse anti-Human, Brilliant Violet 650, Clone: M5E2, BD
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD14
Applications
Flow Cytometry
Conjugate
Brilliant Violet 650
Regulatory Status
RUO
Formulation
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Isotype
IgG2a κ
Purification Method
Affinity Purified
Description
LPS receptor; LPS-R; Myeloid cell-specific leucine-rich glycoprotein
Clone
M5E2
Classification
Monoclonal
Host Species
Mouse
Target Species
Baboon, Canine, Cynomolgus, Human, Rhesus Monkey
Immunogen
Human CD14 Protein
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Description
- The M5E2 monoclonal antibody specifically binds to CD14, a 53–55 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein expressed at high levels on monocytes
- Additionally, the anti-CD14 antibody reacts with interfollicular macrophages, reticular dendritic cells, and some Langerhans cells
- CD14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, LPB
- The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
- This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
- BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
- Due to the excitation and emission characteristics of the acceptor dye, there will bespillover into the APC and Alexa Fluor™ 700 detectors
- However, the spillover can be corrected through compensation as with any other dye combination.