4840433
CD3 Mouse anti-Human, BB515, Clone: UCHT1, BD
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
CD3
Applications
Flow Cytometry
Conjugate
Brilliant Blue 515 (BB515)
Regulatory Status
RUO
Immunogen
Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Clone
UCHT1
Classification
Monoclonal
Host Species
Mouse
Target Species
Human
Isotype
IgG1 κ
Purification Method
Affinity Purified
Description
CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon
Related Products
Description
- The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex
- CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes
- Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody
- CD3 plays a central role in signal transduction during antigen recognition
- The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε
- The antibody was conjugated to BD Horizon™ BB515 which was developed exclusively by BD Biosciences
- With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g
- 530/30-nm filter)
- This dye provides a much brighter alternative to FITC with less spillover into the PE detector.