4747111
CD3 Mouse anti-Human, BUV395, Clone: UCHT1, BD
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
CD3
Applications
Flow Cytometry
Conjugate
Brilliant Ultraviolet 395
Regulatory Status
RUO
Formulation
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Isotype
IgG1 κ
Purification Method
Affinity Purified
Description
CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon
Clone
UCHT1
Classification
Monoclonal
Host Species
Mouse
Target Species
Human
Immunogen
Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Related Products
Description
- The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex
- CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes
- Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody
- CD3 plays a central role in signal transduction during antigen recognition
- The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε
- The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355nm laser equipped instrument
- With an Ex Max at 348nm and an Em Max at 395nm, this dye has virtually no spillover into any other detector
- BD Horizon™ BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.