c-Myc Antibody (9E10) - BSA Free, Novus Biologicals™
Mouse Monoclonal Antibody has been used in 33 publications
Manufacturer: Fischer Scientific
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Antigen
c-Myc
Concentration
1 mg/mL
Applications
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In situ PLA
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Autophagy, Cancer, Cancer Stem Cells, Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, Epigenetics, Epitope Tags, Stem Cell Markers, Transcription Factors and Regulators, Tumor Suppressors
Gene Accession No.
P01106
Gene ID (Entrez)
4609
Immunogen
A synthetic peptide corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) of human c-Myc Antibody (9E10). [UniProt# P01106]
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen
48.8 kDa
Clone
90000000000
Dilution
Western Blot 0.5-2.0 ug/ml, Simple Western 1:200, Flow Cytometry 1:50-1:200. Use reported in scientific literature (PMID 21315712), ELISA 1:100-1:2000, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200, Immunoblotting reported in scientific literature (PMID 23750001), Proximity Ligation Assay reported in scientific literature (PMID 33298911), Sandwich ELISA reported in scientific literature (PMID 33122198), Flow (Intracellular), Chromatin Immunoprecipitation (ChIP)
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse, Bovine, Drosophila
Gene Alias
9E10 anti-Myc, 9E10 Myc, avian myelocytomatosis viral oncogene homolog, BHLHE39, bHLHe39MRTL, Class E basic helix-loop-helix protein 39, c-Myc, MYC, myc proto-oncogene protein, MYCC, myc-related translation/localization regulatory factor, Proto-oncogene c-Myc, Transcription factor p64, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)
Gene Symbols
MYC
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.
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Description
- c-Myc Monoclonal specifically detects c-Myc in Human, Mouse, Bovine, Drosophila samples
- It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Proximity Ligation Assay, Sandwich ELISA, Flow (Intracellular).