50-0M8-5500UG
beta-NGF Monoclonal Antibody, PeproTech®, Invitrogen™
Manufacturer: Pepro Tech Inc
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-0M8-5500UG-Each-of-1 | In Stock | ₹ 16,109.00 |
50-0M8-5500UG - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,109.00
GST (18%): ₹ 2,899.62
Total Price: ₹ 19,008.62
Antigen
beta-NGF
Classification
Monoclonal
Formulation
PBS with no preservative
Gene Accession No.
P01138
Gene Symbols
NGF
Immunogen
E.coli-derived Recombinant Human beta-NGF
Quantity
500 μg
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Applications
ELISA, Western Blot
Conjugate
Unconjugated
Gene
NGF
Gene Alias
B nerve growth factor; beta ngf; beta-nerve growth factor; BetaNGF; beta-NGF; bNGF; b-NGF; HSAN5; MGC161426; MGC161428; nerve growth factor; nerve growth factor (beta polypeptide); nerve growth factor, beta; nerve growth factor, beta polypeptide; nerve growth factor, beta subunit; NGF; NGF b; NGF β; Ngfb; NGFbeta; NGFβ; β nerve growth factor; β NGF; βNGF
Host Species
Mouse
Purification Method
Protein G
Regulatory Status
RUO
Gene ID (Entrez)
4803
Content And Storage
-20°C
Form
Lyophilized
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Description
- AA Sequence of recombinant protein: SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA
- Preparation: Produced in mice immunized with highly pure Recombinant Human beta-NGF
- Anti-Human beta-NGF-specific antibody was purified from cell culture by Protein G affinity chromatography
- Sandwich ELISA: Assuming 100 μL/well, a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 200 pg/mL of Recombinant Human beta-NGF when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human beta-NGF (500-P85BT) as the detection antibody at a concentration of approximately 0.5- 1.0 μg/mL
- Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 μg/mL
- When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human beta-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions
- Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells
- The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites
- Although both types of receptor bind NGF only the high affinity form is capable of signal transduction
- Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex
- Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species
- NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons
- Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons
- Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.