Estrogen Receptor beta Monoclonal Antibody (MC10), eBioscience™, Invitrogen™

Description

• Description: The MC10 monoclonal antibody recognizes all forms of human estrogen receptor (ER) beta
• ER beta is a member of a superfamily of nuclear receptors, which are ligand-dependent transcription factors
• ER beta like ER alpha, becomes activated by binding the hormone estrogen, causing a dissociation from chaperone proteins and subsequent dimerization with either ER beta or ER alpha
• Unlike ER alpha, the result of ER beta signaling is thought to be anti-proliferative and proapoptotic
• The MC10 clone recognizes full length ER beta as well as each of its splice variants
• Applications Reported: This MC10 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, ELISA, and immunocytochemistry
• Applications Tested: This MC10 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using either low or high pH antigen retrieval and can be used at less than or equal to 30 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described
• Human ER beta was initially cloned and characterized from testis
• Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors
• The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA
• When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins
• There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively)
• Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha
• Some ER beta isoforms dominantly inhibit the activity of estrogen receptor alpha in reproductive tissues
• ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.