TCR V beta 11 Monoclonal Antibody (RR3-15), APC, eBioscience™, Invitrogen™

Description

• Description: This RR3-15 monoclonal antibody reacts with the mouse T-cell receptor (TCR) V beta 11 chain
• Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement
• The RR3-15 antibody recognizes the V beta 11 chain on T cells from mouse strains with the b haplotype in the Tcrb gene complex, including C57BL/6 and B10, but is absent in mice with the a haplotype, such as C57L, SJL, and SWR
• In addition, reports indicate that Vbeta11+ T cells are eliminated in mice bearing the MHC Class II I-E molecule
• Finally, V beta 11+CD4+ T cells have been associated with graft-versus-host disease (GVHD) severity
• The RR3-15 antibody has been reported to activate V beta 11+ T cells
• Applications Reported: This RR3-15 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This RR3-15 antibody has been tested by flow cytometric analysis of mouse lymph node cells
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• TCR V beta 11 Monoclonal antibody specifically detects TCR V beta 11 in Mouse samples
• It is validated for Flow Cytometry.