Podoplanin Monoclonal Antibody (eBio8.1.1 (8.1.1)), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• Description: The 8.1.1 monoclonal antibody reacts with mouse podoplanin (T1a, gp38, aggrus), a 43 kDa transmembrane glycoprotein, named for its expression in kidney glomerular epithelial cells (podocytes)
• In addition, Podoplanin is expressed in epithelial and mesothelial cells such as intestinal epithelium, alveolar type I cells, podocytes, and mesothelium of the visceral peritoneum
• It was also shown to be a potent marker for lymphatic endothelium
• Podoplanin is also expressed by subcapsular epithelial cells of the murine thymus
• Mice deficient in Podoplanin die at birth because of a respiratory defect and congenital lymphedema due to a failure in lymphatic pattern formation
• Applications Reported: This eBio8.1.1 (8.1.1) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBio8.1.1 (8.1.1) antibody has been tested by flow cytometric analysis of the TE-71 cell line
• This can be used at less than or equal to 0.125 µg per test
• A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
• 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
• Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight
• Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs
• Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells
• As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes
• Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential
• Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue
• Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells
• Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC
• Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm
• Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.