IL-10 Monoclonal Antibody (JES3-9D7), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10)
• Applications Reported: This JES3-9D7 antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis
• Applications Tested: This JES3-9D7 antibody has been pre-titrated and tested by intracellular staining anf flow cytometryci analysis of Th2 polarized and stimulated human peripheral blood cells
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes
• This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages
• It also enhances B cell survival, proliferation, and antibody production
• Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway
• Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract
• Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis
• Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.