CD319 (CRACC) Monoclonal Antibody (162), PE, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-112-8664-Each-of-1 | In Stock | ₹ 28,836.00 |
50-112-8664 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 28,836.00
GST (18%): ₹ 5,190.48
Total Price: ₹ 34,026.48
Antigen
CD319 (CRACC)
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
Q9NQ25
Gene Symbols
SLAMF7
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
57823
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
162
Conjugate
PE
Gene
SLAMF7
Gene Alias
19A; 19A24; 19A24 protein; 4930560D03Rik; CD2 subset 1; CD2-like receptor activating cytotoxic cells; CD2-like receptor-activating cytotoxic cells; CD319; CRACC; CS1; Leukocyte cell-surface antigen; membrane protein FOAP-12; Novel Ly9; novel LY9 (lymphocyte antigen 9) like protein; protein 19A; RGD1560190; SLAM family member 7; SLAMF7; UNQ576/PRO1138
Host Species
Mouse
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG2b κ
Related Products
Description
- Description: The monoclonal antibody 162 recognizes CRACC (CD2 like receptor for activating cytotoxic cells) also known as CD319 and CS1
- It is a member of the SLAM receptor family
- CRACC is a 66kDa glycosylated transmembrane protein which contains a cytoplasmic ITSM domain (Immunoreceptor Tyrosine-based Switch Motif)
- It is this domain, found in other proteins such as CD244 and NTB-A, that interacts with SH2 containing proteins such as SAP
- Interestingly the ITSM domain in CRACC is SAP independent
- Instead EAT-2 acts as the signaling molecule for CRACC
- This has profound impact for patients with X-linked lymphoproliferative disease (contains a SAP mutation)
- It should be noted that a splice variant has been reported which deletes the ITSM domain
- Applications Reported: This 162 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This 162 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
- This can be used at 5 μL (1 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
- Filtration: 0.2 μm post-manufacturing filtered
- SLAMF7 contains one Ig-like C2-type (immunoglobulin-like) domain
- Isoform 1 mediates NK cell activation through a SAP-independent extracellular signal-regulated ERK-mediated pathway
- It may play a role in lymphocyte adhesion
- Isoform 3 does not mediate any activation
- SAP can bind the cytoplasmic tail of isoform 1 when phosphorylated in the presence of Fyn (in vitro)
- SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea
- Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines
- The isoform 3 is expressed at much lower level than isoform 1
- There are three named isoforms.