IL-12 p35 Monoclonal Antibody (B-T21 (BT21)), eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-112-8691-Each-of-1 | In Stock | ₹ 21,271.00 |
50-112-8691 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 21,271.00
GST (18%): ₹ 3,828.78
Total Price: ₹ 25,099.78
Antigen
IL-12 p35
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P29459
Gene Symbols
IL12A
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
3592
Content And Storage
4° C
Form
Liquid
Applications
ELISA
Clone
B-T21 (BT21)
Conjugate
Unconjugated
Gene
IL12A
Gene Alias
CLMF; CLMF p35; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; il 12; IL-12 subunit p35; IL-12, subunit p35; Il12a; IL-12A; IL12p35; IL-12p35; IL35 subunit; ILN; Interleukin; Interleukin 12; interleukin 12 p35 subunit; interleukin 12, p35; interleukin 12a; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); Interleukin12; interleukin-12 alpha chain; interleukin-12 beta p35 subunit; interleukin-12 p35 subunit; interleukin-12 subunit alpha; Ll12a; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NKSF1; P35
Host Species
Mouse
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1
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Description
- Description: The B-T21 antibody reacts with the p35 subunit of human interleukin-12 (IL-12)
- IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted primarily by activated B cells
- For optimal detection of human IL-12 p70 by ELISA, a combination of purified B-T21 antibody for capture and biotinylated C8.6 antibody for detection is recommended
- Applications Reported: The B-T21 antibody has been reported for use as capture antibody in an IL-12 p70 sandwich ELISA
- Applications Tested: The B-T21 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-12 p70 in combination with the biotinylated C8.6 (13-7129) antibody for detection and recombinant human IL-12 p70 (14-8129) as the standard
- A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 μg/mL
- A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate
- Purity: Greater than 90%, as determined by SDS-PAGE
- Aggregation: Less than 10%, as determined by HPLC
- Filtration: 0.2 μm post-manufacturing filtered
- Interleukin 12A is a novel signaling cytokine belonging to the Interleukin-6 protein superfamily
- Interleukin-12 alpha chain precursor (IL-12A) is a 35-kD subunit of disulfide-linked heterodimer of cytokine IL-12 and has a broad array of biological activities
- Reports suggest that IL-12A can act as a growth factor for activated T and NK cells and enhance the lytic activity of NK/lymphokine-activated Killer cells
- IL-12A stimulates the production of IFN-gamma by resting PBMC as well as T-cell-independent induction of interferon (IFN)-gamma, and helps in the differentiation of both Th1 and Th2 cells
- It is also involved in positive regulation of tyrosine phosphorylation of Stat4 protein
- The signaling process of IL-12A in innate immunity requires nitric oxide synthase 2A
- Along with EBI3, IL-12A forms a novel heterodimeric cytokine, designated as interleukin-35 (IL-35), that may be specifically produced by T reg cells and is required for maximal suppressive activity.