Phycoerythrin Monoclonal Antibody (eBioPE-DLF), Biotin, eBioscience™, Invitrogen™

Description

• Description: The eBioPE-DLF monoclonal antibody reacts with phycoerythrin (PE), PE-Cy5 and PE-Cy7, with no crossreactivity to fluorescein (FITC) or allophycocyanin (APC)
• When using eBioPE-DLF as a second-step antibody, in combination with an appropriate third step, to bind a PE-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated PE antibody
• Therefore no interference or quenching of the PE fluorochrome is observed
• The eBioPE-DLF can be used for separation of cells labeled with PE-conjugated antibodies or staining
• Please note that the optimal concentration for this antibody will vary depending on the density of the primary PE-conjugated antibody and the application tested
• Applications Reported: This eBioPE-DLF antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBioPE-DLF antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.06 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Filtration: 0.2 μm post-manufacturing filtered
• Anti-RPE (R-Phycoerythrin) antibodies can be used to bind conjugated primary antibody without decreasing fluorescent intensity of the RPE fluorochrome
• This method is used for separation of cells labeled with RPE-conjugated antibodies.