CD45.1 Monoclonal Antibody (A20), PE-Cyanine7, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-112-9620-Each-of-1 | In Stock | ₹ 21,983.00 |
50-112-9620 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 21,983.00
GST (18%): ₹ 3,956.94
Total Price: ₹ 25,939.94
Antigen
CD45.1
Classification
Monoclonal
Concentration
0.2 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P06800
Gene Symbols
Ptprc
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
19264
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
A20
Conjugate
PE-Cyanine7
Gene
Ptprc
Gene Alias
B220; Cd45; CD45 antigen; CD45R; GP180; Lca; L-CA; Leukocyte common antigen; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen
Host Species
Mouse
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG2a κ
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Description
- Description: The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains
- The strains that express CD45.1 include SJL/J, DA, STS/A and RIII
- CD45.1 is expressed by all leukocytes in these strains
- Applications Reported: The A20 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This A20 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Light sensitivity: This tandem dye is sensitive photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (cat
- 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
- 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
- Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
- CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes
- CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
- CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains
- These CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
- Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis
- CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.