50-112-9665
CD45.2 Monoclonal Antibody (104), APC-eFluor™ 780, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-112-9665-Each-of-1 | In Stock | ₹ 29,548.00 |
50-112-9665 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 29,548.00
GST (18%): ₹ 5,318.64
Total Price: ₹ 34,866.64
Antigen
CD45.2
Classification
Monoclonal
Concentration
0.2 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P06800
Gene Symbols
Ptprc
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
19264
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
104
Conjugate
APC-eFluor 780
Gene
Ptprc
Gene Alias
B220; Cd45; CD45 antigen; CD45R; GP180; LCA; L-CA; leukocyte common antigen; loc; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; Ptprc; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein
Host Species
Mouse
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG2a κ
Related Products
Description
- Description: The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains
- The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR
- CD45.2 is expressed by all leukocytes in these strains
- Applications Reported: This 104 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This 104 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 1 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm)
- Please make sure that your instrument is capable of detecting this fluorochome
- Light sensitivity: This tandem is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (cat
- 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
- CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes
- CD45.2 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
- CD45.2 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains
- These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
- Besides the role in immunoreceptor signaling, CD45.2 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis
- CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.