50-120-94
CD275 (B7-H2) Monoclonal Antibody (HK5.3), Biotin, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-120-94-Each-of-1 | In Stock | ₹ 23,585.00 |
50-120-94 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 23,585.00
GST (18%): ₹ 4,245.30
Total Price: ₹ 27,830.30
Antigen
CD275 (B7-H2)
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
Q9JHJ8
Gene Symbols
Icosl
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
50723
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
HK5.3
Conjugate
Biotin
Gene
Icosl
Gene Alias
AU044799; B7 homolog 2; B7 homologue 2; B7h; B7H2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP1; B7RP-1; BG071784; CD275; GI50; GL50; GL50-B; ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell costimulator ligand; inducible T-cell co-stimulator ligand; KIAA0653; LICOS; Ly115l; mKIAA0653; RGD1562791; transmembrane protein B7-H2 ICOS ligand
Host Species
Rat
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG2a κ
Related Products
Description
- Description: The HK5.3 monoclonal antibody reacts with mouse B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand
- B7RP-1, a member of the B7 family, has a predicted molecular weight of ∽40 kDa and belongs to the Ig superfamily
- Mouse B7RP-1 is expressed by antigen presenting cells including B cells, monocytes/macrophages and dendritic cells
- B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells
- The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway
- Applications Reported: The HK5.3 antibody has been reported for use in flow cytometric analysis
- HK5.3 is not recommended for use in immunohistochemical staining
- Applications Tested: The HK5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Filtration: 0.2 μm post-manufacturing filtered
- Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS)
- ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function
- ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue
- Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.