CD100 (SEMA4D) Monoclonal Antibody (BMA12 (BMA-12)), eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-127-77-Each-of-1 | In Stock | ₹ 30,883.00 |
50-127-77 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 30,883.00
GST (18%): ₹ 5,558.94
Total Price: ₹ 36,441.94
Antigen
CD100 (SEMA4D)
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
O09126
Gene Symbols
Sema4d
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
20354
Content And Storage
4° C
Form
Liquid
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
Clone
BMA12 (BMA-12)
Conjugate
Unconjugated
Gene
Sema4d
Gene Alias
A8; BB18; C9orf164; CD100; coll-4; GR3; M-Sema G; M-sema-G; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D; sema J; Sema4d; Semacl2; SEMAJ; semaphorin 4D; semaphorin H; semaphorin-4D; Semaphorin-C-like 2; semaphorin-J; Semcl2
Host Species
Rat
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG2a
Related Products
Description
- Description: The BMA12 monoclonal antibody reacts with mouse CD100, also known as Sema4D
- Sema4D/CD100 is a 150kDa transmembrane protein expressed constitutively on T cells and is involved in the activation of B cells through its interaction with CD72
- Interaction of these two proteins diminishes the negative signaling normally effected by CD72
- CD100 is already expressed strongly on resting T cells and somewhat weakly on B cells, but activation upregulates expression on both cell types
- Antigen expression has been reported in Spleen, thymus and lymph nodes
- Applications Reported: The BMA12 (BMA-12) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining
- It has also been reported for use in neutralization of mouse CD100
- (Please use Functional Grade purified BMA12, cat
- 16-1001, in functional assays.)
- Applications Tested: The BMA12 (BMA-12) antibody has been tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 0.25 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Purity: Greater than 90%, as determined by SDS-PAGE
- Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development
- CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro
- CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression
- CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling
- CD100 is expressed on resting and PHA stimulated T cells
- The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.