CD166 (ALCAM) Monoclonal Antibody (eBioALC48), eBioscience™, Invitrogen™

Description

• Description: Activated leukocyte cell adhesion molecule (ALCAM; CD166) is a 105 kD cell-surface adhesion molecule belonging to the Ig superfamily of receptors
• ALCAM is a type I transmembrane glycoprotein, possessing five extracellular Ig domains, a transmembrane domain, and a short cytoplasmic tail
• ALCAM is involved in homotypic adhesion, as well as heterotypic adhesion with its natural ligand, CD6
• A soluble isoform of ALCAM has been shown to inhibit homophilic adhesive interactions
• ALCAM is expressed on a variety of cell types, including activated T and B cells, monocyte-derived dendritic cells, and thymic epithelial cells
• ALCAM expression has also been used as a marker for the isolation of pluripotent stem cells
• Applications Reported: This eBioALC-48 antibody has been reported for use in flow cytometric analysis and immunohistochemistry on paraffin-embedded tissue sections
• Applications Tested: The eBioALC-48 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain
• This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration
• Multiple alternatively spliced transcript variants encoding different isoforms have been found.