CD253 (TRAIL) Monoclonal Antibody (N2B2), eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-133-51-Each-of-1 | In Stock | ₹ 22,695.00 |
50-133-51 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 22,695.00
GST (18%): ₹ 4,085.10
Total Price: ₹ 26,780.10
Antigen
CD253 (TRAIL)
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P50592
Gene Symbols
TNFSF10
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
22035
Content And Storage
4° C
Form
Liquid
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Neutralization
Clone
N2B2
Conjugate
Unconjugated
Gene
TNFSF10
Gene Alias
A330042I21Rik; AI448571; Apo-2 ligand; APO2L; Apo-2L; CD253; CD253 antigen; chemokine tumor necrosis factor ligand superfamily member 10; Ly81; Protein TRAIL; TL2; TN; TNF superfamily member 10; TNF-related apoptosis inducing ligand; TNF-related apoptosis inducing ligand TRAIL; TNF-related apoptosis-inducing ligand; Tnfsf10; TNLG6A; TRAIL; TRAIL protein; TRAIL/APO2L; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor (ligand) superfamily member 10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; tumor necrosis factor ligand 6A; tumor necrosis factor ligand superfamily member 10; tumor necrosis factor superfamily member 10
Host Species
Rat
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG2a κ
Related Products
Description
- Description: The N2B2 monoclonal antibody reacts with mouse TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily
- TRAIL is not detected on the surface of freshly isolated T, B, or NK cells, but can be induced preferentially on CD3^- NK1.1^+ NK cells after stimulation with IL-2 or IL-15
- N2B2 inhibits IL-2- or IL-15-activated NK cell cytotoxicity against mouse fibrosarcoma L929 target cells
- Applications Reported: N2B2 can be used in flow cytometric analysis, and blocking of TRAIL in functional assays
- (Please use Functional Grade purified N2B2, cat
- 16-5951, in functional assays.)
- Applications Tested: The N2B2 antibody has been tested by flow cytometric analysis of mouse TRAIL transfected cells and can be used at less than or equal to 1 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Purity: Greater than 90%, as determined by SDS-PAGE
- Aggregation: Less than 10%, as determined by HPLC
- Filtration: 0.2 μm post-manufacturing filtered
- TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family
- TRAIL preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues
- TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG
- The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis
- The binding of TRAIL protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3
- Receptors for TRAIL include two death domain containing receptors, death receptor 4 (DR4) and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain
- TRAIL is a type II membrane protein and expressed in a variety of human tissues
- Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.