MCL-1 Monoclonal Antibody (Ab22), eBioscience™, Invitrogen™

Description

• Description: This Ab22 monoclonal antibody reacts with the human myeloid cell leukemia-1 (Mcl-1) protein
• This 40 kDa Bcl-2 family member is expressed in B and T cells, macrophages, neutrophils, and hematopoietic stem cells
• Mcl-1 is also expressed in non-immune cells such as fibroblasts, epithelial cells, neuroendocrine cells, chondrocytes, and hepatocytes
• Reportedly induced by growth factors and cytokines in normal cells, Mcl-1 expression of Mcl-1 can be dysregulated in various leukemias and cancer
• Mcl-1 mediates cell survival and proliferation by sequestering the pro-apoptotic Bcl-2 proteins Bak, Bax, and Bim
• This protein is also involved in germinal center and memory B cell formation, as well as in myeloid and early lymphoid differentiation
• Finally, Mcl-1 activity and dimerization with Bcl-2 family members is dependent on serine and threonine phosphorylation
• Applications Reported: This Ab22 antibody has been reported for use in immunoblotting (WB) and immunocytochemistry (ICC)
• Applications Tested: This Ab22 antibody has been tested by immunocytochemistry on fixed and permeabilized MCF7 cells at less than or equal to 5 μg/mL
• This Ab22 has also been tested by western blot of reduced Jurkat cell lysate and can be used at 5 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• MCL1 (Myeloid cell leukemia-1) belongs to the Bcl-2 family and is involved in programing, differentiation and concomitant maintenance of cell viability, but not of proliferation
• Isoform 1 of MCL1 inhibits apoptosis while isoform 2 promotes it
• The carboxy terminal of MCL1 and bcl-2 share significant sequence homology
• Expression of MCL1 is increased upon exposure of ML-1 cells to various types of DNA damaging agents (e.g
• ionizing radiation, μgtraviolet radiation, and alkylating drugs) along with increases in GADD45 and Bax and a decrease in bcl-2
• Enhanced expression of MCL1, prominently associated with mitochondria, complements the continued expression of bcl-2 in ML-1 cells undergoing differentiation
• Like bcl-2, MCL1 has the capacity to promote cell viability under conditions that otherwise cause apoptosis
• While the mechanism by which MCL1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak
• MCL1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types
• MCL1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals
• MCL1 exists as at least two distinct isoforms designated MCL1L and MCL1S
• In marked contrast to the larger isoform of MCL1, overexpression of MCL1S promotes cell death.