CD2 Monoclonal Antibody (RPA-2.10), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells
• CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells
• RPA-2.10 blocks mixed lymphocyte reaction
• RPA-2.10 crossreacts to non-human primates and pigs
• Applications Reported: The RPA-2.10 antibody been reported for use in flow cytometric analysis
• RPA-2.10 has also been reported in blocking of CD2 activity in in vitro functional assays
• Applications Tested: This RPA-2.10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• CD2 (LFA-2) is a monomeric surface antigen (MW range 45-58 kDa) of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells
• CD2 is one of the earliest T-cell markers, being present on more than 95% of thymocytes and it is also found on some natural killer cells, but not on B lymphocytes
• Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it
• The interaction between CD2 and CD58 stabilizes adhesion between T cells and antigen presenting or target cells
• Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion
• Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58
• Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion
• CD2 deficiency reduces intestinal inflammation and helps to control infection
• Diseases associated with CD2 dysfunction include penis squamous cell carcinoma and immune deficiency due to the absence of the thymus.