CD14 Monoclonal Antibody (61D3), Functional Grade, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-142-36-Each-of-1 | In Stock | ₹ 26,077.00 |
50-142-36 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 26,077.00
GST (18%): ₹ 4,693.86
Total Price: ₹ 30,770.86
Antigen
CD14
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
PBS with no preservative; pH 7.2
Gene Accession No.
P08571
Gene Symbols
Cd14
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
929
Content And Storage
4° C
Form
Liquid
Applications
Flow Cytometry, Functional Assay
Clone
61D3
Conjugate
Functional Grade
Gene
Cd14
Gene Alias
CD 14; CD14; CD14 antigen; CD14 molecule; cd14 monocyte; lipopolysaccharide receptor; lipoprotein receptor; LPSR antibody; monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; monocyte differentiation antigen CD14; LOW QUALITY PROTEIN: monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein; myeloid membrane glycoprotein precursor; sCD14; soluble CD14
Host Species
Mouse
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1 κ
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Description
- Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein
- CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells
- Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14
- Applications Reported: The 61D3 antibody has been reported for use in flow cytometric analysis
- 61D3 has also been reported for in vitro functional studies
- (Fluorochrome-conjugated 61D3 is recommended for use in flow cytometry)
- Applications Tested: The 61D3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
- This can be used at less than or equal to 1 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Storage and handling: Use in a sterile environment
- Filtration: 0.2 μm post-manufacturing filtered
- Purity: Greater than 90%, as determined by SDS-PAGE
- Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
- Aggregation: Less than 10%, as determined by HPLC
- CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils
- CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form
- CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein
- The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles
- Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes
- Alternative splicing results in multiple transcript variants encoding the same CD14 isoform
- Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.