50-155-7259
KAP-1 Rabbit anti-Human, Mouse, Polyclonal, Bethyl Laboratories
Manufacturer: Bethyl Laboratories, Inc
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-155-7259-Each-of-1 | In Stock | ₹ 43,546.81 |
50-155-7259 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 43,546.81
GST (18%): ₹ 7,838.426
Total Price: ₹ 51,385.236
Antigen
KAP-1
Classification
Polyclonal
Conjugate
Unconjugated
Gene
TRIM28
Gene Alias
Transcription intermediary factor 1-beta, Tripartite motif protein 28, Nuclear corepressor KAP-1, KRAB-associated protein 1, KAP-1, KRAB-interacting p
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
10155, 21849
Content And Storage
4° C
Form
Liquid
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Concentration
1 mg/ml
Formulation
phosphate, tris citrate with 0.09% sodium azide; pH 7-8
Gene Accession No.
Q13263, Q62318
Gene Symbols
Trim28
Immunogen
Between 1 and 50
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse
Product Type
Antibody
Isotype
IgG
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Description
- The recommended shelf life for this product is 1 year from date of receipt
- Application Note: For ICC, formaldehyde fixation is recommended
- Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors
- There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome
- These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1)
- KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger
- KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression
- KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1)
- KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression
- Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.