CD284 (TLR4) Monoclonal Antibody (UT41), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: The UT41 monoclonal antibody was generated against BaF3 cells overexpressing mouse TLR4
• So far, at least twelve members of the Toll family have been identified
• This family of type I transmembrane protein is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor
• Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation
• TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling
• TLR4, which is approximately 100 kDa, is expressed by myeloid lineage cells
• The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (∽30 kDa) and TLR4 (∽100 kDa), and preferentially reacts with TLR4 that is associated with MD-2
• In comparison, binding of the UT41 monoclonal antibody occurs with and without formation of the TLR4/MD-2 complex
• Please contact eBioscience Technical Support for further information
• Applications Reported: This UT41 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This UT41 antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• TLR4 is a member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity
• TLRs are highly conserved from Drosophila to humans and share structural and functional similarities
• This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes
• It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria.