50-194-3774
Biotin Ligase Epitope TagRabbit anti-Tag, Polyclonal, Rockland™
Manufacturer: Rockland Immunochemicals
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-194-3774-Each-of-1 | In Stock | ₹ 44,948.56 |
50-194-3774 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 44,948.56
GST (18%): ₹ 8,090.741
Total Price: ₹ 53,039.301
Antigen
Biotin Ligase Epitope Tag
Classification
Polyclonal
Conjugate
Unconjugated
Host Species
Rabbit
Quantity
100 μL
Primary or Secondary
Primary
Product Type
Antibody
Target Molecule
Tag
Applications
ELISA, Western Blot
Concentration
70 mg/mL
Formulation
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen
Anti-BIOTIN Ligase Epitope Tag antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide GLNDIFEAQKIEWH (minimal BirA substrate; biotin is added to the sequence at the lysine.)
Regulatory Status
RUO
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Form
Liquid
Related Products
Description
- Store vial at -20°C prior to opening
- Aliquot contents and freeze at -20°C or below for extended storage
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- This antiserum detects the minimal peptide substrate (GLNDIFEAQKIEWH) for the E
- coli biotin ligase tag (BLT of BirA)
- Western blot analysis of B
- subtilis strains producing either (BAP-tagged) SPoIIIAH (XWB49) or (BAP-tagged) SpoIIQ (XWB46) showed strong and specific reactivity to the antisera
- Minor background bands of unknown identity may be observed
- The antisera will recognize the BAP/BLT tag irrespective of the whether the tag is biotinylated (data not shown)
- The peptide is also called the biotin acceptor peptide (BAP) of the biotin carboxyl carrier protein (BCCP) subunit of acetyl CoA carboxylase of E
- coli.