Phospho-E2F-1 (Ser364)Rabbit anti-Chimpanzee, Human, Polyclonal, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This affinity purified antibody is directed against the phosphorylated form of human E2F-1 at the pS364 residue
• Reactivity occurs against human E2F-1 pS364 protein and the antibody is specific for the phosphorylated form of the protein
• Reactivity with non-phosphorylated human E2F-1 is minimal by ELISA
• The antibody does not cross-react with E2F-1 phosphorylated at other sites
• A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence
• Cross reactivity with E2F-1 homologues from other sources has not been determined
• The protein encoded by this gene is a member of the E2F family of transcription factors
• The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses
• The E2F proteins contain several evolutionally conserved domains found in most members of the family
• These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain
• This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain
• This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner
• It can mediate both cell proliferation and p53-dependent/independent apoptosis.