EOMES Monoclonal Antibody (Dan11mag), eBioscience™, Invitrogen™

Description

• Description: This Dan11mag antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2)
• Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates
• In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells
• Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R
• Applications Reported: This Dan11mag antibody has been reported for use in immunohistochemistry of formalin-fixed paraffin embedded tissue sections, intracellular staining followed by flow cytometric analysis, and western blotting
• (Fluorochrome conjugated Dan11mag is recommended for use in intracellular flow cytometry.)
• Applications Tested: This Dan11mag antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse splenocytes and/or western blotting at 5 μg/mL
• This Dan11mag antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low pH antigen retrieval at less than or equal to 5 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box
• T-box genes encode transcription factors involved in the regulation of developmental processes
• A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation
• TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.