beta-NGF Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-271-8441-Each-of-1 | In Stock | ₹ 1,75,552.50 |
50-271-8441 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,75,552.50
GST (18%): ₹ 31,599.45
Total Price: ₹ 2,07,151.95
Antigen
beta-NGF
Classification
Polyclonal
Conjugate
Biotin
Gene
NGF
Gene Alias
B nerve growth factor; beta ngf; beta-nerve growth factor; BetaNGF; beta-NGF; bNGF; b-NGF; HSAN5; MGC161426; MGC161428; nerve growth factor; nerve growth factor (beta polypeptide); nerve growth factor, beta; nerve growth factor, beta polypeptide; nerve growth factor, beta subunit; NGF; NGF b; NGF β; Ngfb; NGFbeta; NGFβ; β nerve growth factor; β NGF; βNGF
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
4803
Content And Storage
-20°C
Isotype
IgG
Applications
Western Blot, ELISA
Concentration
0.1-1.0 mg/mL
Formulation
PBS with no preservative
Gene Accession No.
P01138
Gene Symbols
NGF
Immunogen
E.coli-derived, 27.0 kDa Recombinant Human beta-NGF
Quantity
500μg
Primary or Secondary
Primary
Target Species
Human
Form
Lyophilized
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Description
- AA Sequence of recombinant protein: SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA
- Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human beta-NGF
- Anti-Human beta-NGF-specific antibody was purified by affinity chromatography and then biotinylated
- Sandwich ELISA: To detect Human beta-NGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
- This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human beta-NGF (500-P85) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human beta-NGF
- Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
- When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human beta-NGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
- 500-P85BT-1MG will be provided as 2 x 500 μg Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells
- The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites
- Although both types of receptor bind NGF only the high affinity form is capable of signal transduction
- Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex
- Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species
- NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons
- Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons
- Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.